UBC CPD eLearning

CRISPRa (CRISPR activation) system is a technology based on the improved CRISPR/Cas9 system that uses a synthetic programmable transcription factor to initiate endogenous transactivation of genes. In mammalian cells, the expressing of dCas9 (a nuclease-deficient Cas9 only retaining DNA binding activity) fused with a transcriptional activation domain, and gRNA complementary to the target promoter region in the genome, will result in increased transcription of the corresponding gene.

CRISPR/Cas9 Platform^CB, with many years of experience in gene editing, has recently introduced custom CRISPRa cell line service to help researchers achieve transcriptional activation of target genes by dCas9-VPR system in the shortest period of time.

• dCas9-VPR system

In the dCas9-VPR protein, the activation domains of the p65 subunit of NFκB and Epstein−Barr virus R Transactivator (Rta) are added to the C-terminus of dCas9-Vp64, and the expression of the targeted gene is increased to a greater extent than the three transcription factors compared to dCas9-Vp64.