UBC CPD eLearning

In the realm of drug discovery, understanding the metabolic fate of compounds is essential. Metabolite profiling and identification play a crucial role in deciphering how drugs are transformed in the body. This process not only aids in characterizing the pharmacokinetics of potential therapies but also addresses safety and efficacy before human applications.

The Importance of Metabolite Identification

The identification of drug metabolites derived from both in vitro and in vivo studies is paramount in the discovery process. Such data provides insights into how compounds are biotransformed, the specific metabolic sites, and potential variations across species. This knowledge serves multiple purposes, including optimizing drug structures to enhance bioavailability and pharmacokinetic profiles. Moreover, recognizing species-specific metabolism can guide the selection of appropriate preclinical models.

As compounds progress from discovery to development phases, the demand for accurate and detailed metabolite information intensifies. Not only does it facilitate a thorough understanding of candidate drugs in humans, but it also fulfills regulatory requirements essential for successful drug registration.

Advanced Capabilities at Creative Bioarray

Creative Bioarray is at the forefront of metabolite profiling and identification. Our team brings extensive expertise in the structural characterization and identification of metabolites using cutting-edge technologies and robust analytical methods. The services provided encompass both in vitro and in vivo studies, ensuring a comprehensive analysis of the metabolic pathways.

Metabolite Characterization Services

Our suite of metabolite characterization capabilities is designed to meet diverse research needs:

• Customized study designs based on structure-specific requirements.
• Utilization of a range of in vitro systems, including hepatocytes, S9 fractions, microsomes, and recombinant enzymes.
• Adoption of various chemical synthesis or biocatalytic approaches to produce target metabolites.
• Optimization of reaction conditions to maximize metabolite yield and abundance.
• Small-scale reactions that allow comparison of metabolite profiles via advanced LC/MS/MS techniques.
• Comprehensive data reviews conducted by experienced scientists to ensure high accuracy.

Technical Proficiencies

Our state-of-the-art technical capabilities include:

• High-performance liquid chromatography (HPLC/UPLC)
• Liquid chromatography-mass spectrometry (LC-MS and LC-MS/MS)
• Gas chromatography-mass spectrometry (GC-MS)
• Radiometric profiling and advanced mass spectrometry techniques such as TOF, MALDI-TOF, and LTQ Orbitrap
• Ion mobility spectrometry (IMS) and nuclear magnetic resonance (NMR) spectroscopy

With our high-resolution mass spectrometry metabolite identification services, we deliver critical information on compound metabolism, with turnaround times as short as two weeks. We offer tailored method development or the option to employ existing in-house analytical parameters, catering to varying research requirements.

Conclusion

Metabolite profiling and identification are indispensable in the drug discovery process. From enhancing the understanding of pharmacokinetics to ensuring compliance with regulatory standards, the significance cannot be overstated. With Creative Bioarray’s advanced capabilities and expert personnel, the complexities of metabolite analysis are addressed with precision and reliability.

For inquiries or detailed information about our services, feel free to contact our customer service representatives, available around the clock. Embrace the future of drug discovery with Creative Bioarray, where innovative science meets comprehensive support.